Quantitative Analysis of 17 .BETA.-Estradiol in River Water by Fluorometric Enzyme Immunoassay Using Biotinylated Estradiol
نویسندگان
چکیده
منابع مشابه
New fluorometric enzyme immunoassay for 17beta-estradiol by homogeneous reaction using biotinylated estradiol.
A new fluorometric enzyme immunoassay for 17beta-estradiol (E2) using biotinylated estradiol (BE) as a probe ligand, is described. In this method, E2 is detected indirectly by a solid-phase avidin-biotin binding assay, in which the biotin is immobilized on a microtiter plate (biotin-plate). After the competitive reaction between E2 and BE for the anti-E2 antibody in solution, the free E2 and BE...
متن کاملMeasurement of plasma estradiol-17 beta by solid-phase chemiluminescence immunoassay.
We describe a simple, solid-phase chemiluminescence immunoassay for the measurement of estradiol-17 beta in extracts of peripheral venous plasma. The method has similar sensitivity, specificity, precision, and accuracy to a conventional radioimmunoassay with a tritiated antigen. An immunoglobulin G fraction od monoclonal antibodies to estradiol-6-carboyxmethyl oxime-bovine serum albumin is pass...
متن کامل17 Beta-Estradiol, Integrins, and Synaptic Proteins
ACKNOWLEDGEMENTS Foremost, I would like to sincerely express my gratitude to my mentor Dr. James Simpkins for his continued support, encouragement, sound advice and for his strategic ideas that contributed to the betterment of my thesis work and helped me meet my timelines. This work would not have been possible without him. Also, I would like to sincerely appreciate Dr. Simpkins for his kind a...
متن کاملThe presence of 17-beta estradiol in Mytilus edulis gonadal tissues: evidence for estradiol isoforms.
OBJECTIVES In earlier studies, we demonstrate that 17-beta -estradiol and an estrogen cell surface receptor can be found on various human cells, i.e., vascular endothelial, monocytes, and granulocytes, where they are coupled to nitric oxide release. We further demonstrated this phenomenon in the marine mussel Mytilus edulis ganglionic tissues. In the present report we sought to determine if est...
متن کاملThe Presence of 17 Beta-Estradiol in the Environment: Health Effects and Increasing Environmental Concerns
Endocrine-disrupting compounds (EDCs) as active biological compounds can pose a threat to the environment through acute and chronic toxicity in organisms, accumulation in the ecosystem, and loss of habitats and biodiversity. They also have a range of possible adverse effects on environmental...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2005
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.21.219